Journal: Annals of Dermatology
Article Title: Curcumin Enhances the Anticancer Effects of Binimetinib on Melanoma Cells by Inducing Mitochondrial Dysfunction and Cell Apoptosis with Necroptosis
doi: 10.5021/ad.22.200
Figure Lengend Snippet: Effects of CCM and MEK162 on cell viability, cell morphology, and nuclear morphology. (A) Chemical structure of CCM, (B) chemical structure of MEK162, (C) cell viability was measured by MTT assay in HEMn-MP, G361, and SK-MEL-2 cells. Cells were treated with various concentrations of CCM (0, 5, 10, 20, 40, 80, or 160 µM) or MEK162 (0.25, 0.5, 1, 2, 4, or 8 µM). (D) Cell morphology was examined under phase-contrast microscopy in HEMn-MP, G361, and SK-MEL-2 cells. (E) Nuclear morphology was examined with a fluorescence microscope following DAPI (2 µg/ml) staining in HEMn-MP, G361, and SK-MEL-2 cells. Cells were treated with CCM (10 µM) or MEK162 (0.5 µM), singly or in combination. CCM: curcumin, MEK162: binimetinib, HEMn-MP: human epidermal melanocytes, neonatal, moderately pigmented, DAPI: 4′,6-diamidino-2-phenylindole, Conc.: concentration.
Article Snippet: We purchased human epidermal melanocytes (HEMn-MP) from Cascade Biologics and two human MM cell lines (G361 and SK-MEL-2) from the American Type Culture Collection.
Techniques: MTT Assay, Microscopy, Fluorescence, Staining, Concentration Assay