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hemn-mp #c1025c  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hemn-mp #c1025c
    Hemn Mp #C1025c, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hemn-mp #c1025c/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    hemn-mp #c1025c - by Bioz Stars, 2026-03
    90/100 stars

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    Effects of CCM and MEK162 on cell viability, cell morphology, and nuclear morphology. (A) Chemical structure of CCM, (B) chemical structure of MEK162, (C) cell viability was measured by MTT assay in <t>HEMn-MP,</t> G361, and SK-MEL-2 cells. Cells were treated with various concentrations of CCM (0, 5, 10, 20, 40, 80, or 160 µM) or MEK162 (0.25, 0.5, 1, 2, 4, or 8 µM). (D) Cell morphology was examined under phase-contrast microscopy in HEMn-MP, G361, and SK-MEL-2 cells. (E) Nuclear morphology was examined with a fluorescence microscope following DAPI (2 µg/ml) staining in HEMn-MP, G361, and SK-MEL-2 cells. Cells were treated with CCM (10 µM) or MEK162 (0.5 µM), singly or in combination. CCM: curcumin, MEK162: binimetinib, HEMn-MP: <t>human</t> <t>epidermal</t> melanocytes, neonatal, moderately pigmented, DAPI: 4′,6-diamidino-2-phenylindole, Conc.: concentration.
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    Effects of CCM and MEK162 on cell viability, cell morphology, and nuclear morphology. (A) Chemical structure of CCM, (B) chemical structure of MEK162, (C) cell viability was measured by MTT assay in <t>HEMn-MP,</t> G361, and SK-MEL-2 cells. Cells were treated with various concentrations of CCM (0, 5, 10, 20, 40, 80, or 160 µM) or MEK162 (0.25, 0.5, 1, 2, 4, or 8 µM). (D) Cell morphology was examined under phase-contrast microscopy in HEMn-MP, G361, and SK-MEL-2 cells. (E) Nuclear morphology was examined with a fluorescence microscope following DAPI (2 µg/ml) staining in HEMn-MP, G361, and SK-MEL-2 cells. Cells were treated with CCM (10 µM) or MEK162 (0.5 µM), singly or in combination. CCM: curcumin, MEK162: binimetinib, HEMn-MP: <t>human</t> <t>epidermal</t> melanocytes, neonatal, moderately pigmented, DAPI: 4′,6-diamidino-2-phenylindole, Conc.: concentration.
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    Summary of HLA-A2 status in neonatal melanocyte lines
    Hemn Mp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of CCM and MEK162 on cell viability, cell morphology, and nuclear morphology. (A) Chemical structure of CCM, (B) chemical structure of MEK162, (C) cell viability was measured by MTT assay in HEMn-MP, G361, and SK-MEL-2 cells. Cells were treated with various concentrations of CCM (0, 5, 10, 20, 40, 80, or 160 µM) or MEK162 (0.25, 0.5, 1, 2, 4, or 8 µM). (D) Cell morphology was examined under phase-contrast microscopy in HEMn-MP, G361, and SK-MEL-2 cells. (E) Nuclear morphology was examined with a fluorescence microscope following DAPI (2 µg/ml) staining in HEMn-MP, G361, and SK-MEL-2 cells. Cells were treated with CCM (10 µM) or MEK162 (0.5 µM), singly or in combination. CCM: curcumin, MEK162: binimetinib, HEMn-MP: human epidermal melanocytes, neonatal, moderately pigmented, DAPI: 4′,6-diamidino-2-phenylindole, Conc.: concentration.

    Journal: Annals of Dermatology

    Article Title: Curcumin Enhances the Anticancer Effects of Binimetinib on Melanoma Cells by Inducing Mitochondrial Dysfunction and Cell Apoptosis with Necroptosis

    doi: 10.5021/ad.22.200

    Figure Lengend Snippet: Effects of CCM and MEK162 on cell viability, cell morphology, and nuclear morphology. (A) Chemical structure of CCM, (B) chemical structure of MEK162, (C) cell viability was measured by MTT assay in HEMn-MP, G361, and SK-MEL-2 cells. Cells were treated with various concentrations of CCM (0, 5, 10, 20, 40, 80, or 160 µM) or MEK162 (0.25, 0.5, 1, 2, 4, or 8 µM). (D) Cell morphology was examined under phase-contrast microscopy in HEMn-MP, G361, and SK-MEL-2 cells. (E) Nuclear morphology was examined with a fluorescence microscope following DAPI (2 µg/ml) staining in HEMn-MP, G361, and SK-MEL-2 cells. Cells were treated with CCM (10 µM) or MEK162 (0.5 µM), singly or in combination. CCM: curcumin, MEK162: binimetinib, HEMn-MP: human epidermal melanocytes, neonatal, moderately pigmented, DAPI: 4′,6-diamidino-2-phenylindole, Conc.: concentration.

    Article Snippet: We purchased human epidermal melanocytes (HEMn-MP) from Cascade Biologics and two human MM cell lines (G361 and SK-MEL-2) from the American Type Culture Collection.

    Techniques: MTT Assay, Microscopy, Fluorescence, Staining, Concentration Assay

    Summary of HLA-A2 status in neonatal melanocyte lines

    Journal: Journal of Translational Medicine

    Article Title: Cytotoxic T lymphocyte responses against melanocytes and melanoma

    doi: 10.1186/1479-5876-9-122

    Figure Lengend Snippet: Summary of HLA-A2 status in neonatal melanocyte lines

    Article Snippet: Melanocyte line HeMn-MP 4C0197 was purchased from Cascade Biologics (Portland, Oregon), and lines HeMn-LP and HeMn-MP with lot numbers 3C0523, 3C0527, 3C0651, 3C0659, 3C0764, and 3C0661 were kindly provided by Dr. Gary Shipley (Cascade Biologics).

    Techniques:

    Representative FACS plot showing degranulation in HLA-A*0201-restricted gp100-specific CTL clones . CD107 mobilization quantification in gp100-specific, (A) high RE, and (B) intermediate RE CTL clones upon activation by target melanoma and melanocyte lines. CTL clones demonstrated MHC-restricted, peptide specific response against target cells with RE corresponding to levels as previously described . All melanoma cell lines are HLA-A*0201-positive; melanocyte lines 4C0197 and 3C0661 are A*0201-positive while 3C0659 and 3C074 are A*0201-negative.

    Journal: Journal of Translational Medicine

    Article Title: Cytotoxic T lymphocyte responses against melanocytes and melanoma

    doi: 10.1186/1479-5876-9-122

    Figure Lengend Snippet: Representative FACS plot showing degranulation in HLA-A*0201-restricted gp100-specific CTL clones . CD107 mobilization quantification in gp100-specific, (A) high RE, and (B) intermediate RE CTL clones upon activation by target melanoma and melanocyte lines. CTL clones demonstrated MHC-restricted, peptide specific response against target cells with RE corresponding to levels as previously described . All melanoma cell lines are HLA-A*0201-positive; melanocyte lines 4C0197 and 3C0661 are A*0201-positive while 3C0659 and 3C074 are A*0201-negative.

    Article Snippet: Melanocyte line HeMn-MP 4C0197 was purchased from Cascade Biologics (Portland, Oregon), and lines HeMn-LP and HeMn-MP with lot numbers 3C0523, 3C0527, 3C0651, 3C0659, 3C0764, and 3C0661 were kindly provided by Dr. Gary Shipley (Cascade Biologics).

    Techniques: Clone Assay, Activation Assay

    Relative ratio of TAA mRNA expression in each target cell compared to glyceraldehye-3-phosphate dehydrogenase.

    Journal: Journal of Translational Medicine

    Article Title: Cytotoxic T lymphocyte responses against melanocytes and melanoma

    doi: 10.1186/1479-5876-9-122

    Figure Lengend Snippet: Relative ratio of TAA mRNA expression in each target cell compared to glyceraldehye-3-phosphate dehydrogenase.

    Article Snippet: Melanocyte line HeMn-MP 4C0197 was purchased from Cascade Biologics (Portland, Oregon), and lines HeMn-LP and HeMn-MP with lot numbers 3C0523, 3C0527, 3C0651, 3C0659, 3C0764, and 3C0661 were kindly provided by Dr. Gary Shipley (Cascade Biologics).

    Techniques: Expressing